Livestock inspectors Gene Autry and Frog Millhouse are accused of favoritism when they try to help an old rancher and his attractive daughter whose cattle appear have hoof and mouth disease and are doomed to be destroyed. With the aid of a friendly elephant, Gene and Frog uncover the truth. Gene Autry and the Rodeoliers sing "I'm Gonna Round Up My Blues." Gene and June Storey sing "Moonlight on the Ranch House." Gene sings "Big Bull Frog." Smiley Burnette sings "There's Nothing Like Work."
Hired as a radio singer, singing cowboy Gene Autry soon discovers the show's sponsor is a crooked oil company cheating, among others, an orphanage on the Mexican border. With the aid of a music-loving bandit, Gene and Smiley Burnette ride to the rescue.
When Deputy Sheriff Gene Autry agrees to become the guardian of a dying friend's three young sons from Chicago, he soon learns that the boys are a pack of ruffians and not the least bit impressed with cowboys and ranch life.
Ranch foreman Gene Autry, with the help of Frog Millhouse (Smiley Burnette), turns the Circle J Ranch into a dude ranch to help an old friend fool his daughter's rich fiancé into believing he is a wealthy rancher.